Nanopore back titration analysis of dipicolinic acid
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منابع مشابه
Nanopore sensors for nucleic acid analysis.
Nanopore analysis is an emerging technique that involves using a voltage to drive molecules through a nanoscale pore in a membrane between two electrolytes, and monitoring how the ionic current through the nanopore changes as single molecules pass through it. This approach allows charged polymers (including single-stranded DNA, double-stranded DNA and RNA) to be analysed with subnanometre resol...
متن کاملDipicolinic acid-less mutants of Bacillus cereus.
When J. Powell (Biochem. J. 54:210, 1953) established dipicolinic acid (DPA) as an important constituent of bacterial spores, microbiologists became concerned about the function this compound serves and about its biosynthetic pathway. It is now generally considered that DPA is involved in the heat resistance of spores, but the mechanism is unknown. A number of schemes have been proposed for its...
متن کاملBiosynthesis of dipicolinic acid in Bacillus megaterium.
Very little is known of the precursors of the dipicolinic acid (pyridine-2,6-dicarboxylic acid) in bacterial spores. C'4-2,6-diaminopimelic acid was shown to contribute to the formation of dipicolinic acid in Bacillus cereus var. mycoides (Perry and Foster, 1955; Foster, 1956) but the efficiency of the conversion was low, possibly because of a slow penetration of the cells by the exogenously su...
متن کاملDipicolinic acid synthesis in Penicillium citreo-viride.
Hodson, Phillip H. (University of Texas, Austin), and J. W. Foster. Dipicolinic acid synthesis in Penicillium citreo-viride. J. Bacteriol. 91:562-569. 1966.-Dipicolinic acid (DPA) accumulation in culture filtrates of the mold Penicillium citreo-viride was studied in surface and submerged cultures. Good DPA yields were obtained in suspensions of washed, submerged mycelium in the presence of a ca...
متن کاملSpectroscopic Study of Dipicolinic Acid Interaction with Bovine Serum Albumin
Spectral changes of bovine serum albumin (BSA) in the presence of dipicolinic acid (DPA) were studied with UV/Vis absorption and fluorescence spectroscopy. The change in UV/vis spectra of BSA in the presence of DPA indicated the protein weakly interacting with DPA. The DPA quenching to the fluorescence of BSA leads to the calculation of the BAS-DPA association constant K of 8.9 x 104 Lmol-1 at ...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2014
ISSN: 0173-0835
DOI: 10.1002/elps.201400255